Figure 3.
Effect of individual and combined osmotic, heat and Pseudomonas syringae pv. tomato DC3000 (Pst) treatments on the expression of AtPR-1 (A), AtPR-5 (B) and AtTN-13 (C) genes in Arabidopsis (Col-0) plants. Pst was applied 16 h after the abiotic stress treatments. Leaves were sampled 48 h after MgCl2 (mock) or Pst treatments. O: osmotic stress, H: heat. Data are represented as log2 fold change, normalized with reference gene (plant biomass expressed protein, expG) and relative to the mock-treated control. The zero-line represents MgCl2-infiltrated (mock) plants. Bars represent the mean and standard deviation of three biological replicates. Asterisks indicate significant differences, 1-way ANOVA; post-hoc LSD, * p < 0.05.