Table 1.
Commercially available ELISA kits used for the diagnosis of SRLV infections.
| Commercial Kit Product Name | ELISA Format | Antigen | Sample/ Diagnostic Matrix |
Se/Sp | Reference Test | Ref |
|---|---|---|---|---|---|---|
| LSIVetTM Ruminant Maedi-Visna/CAEV serum ELISA kit (LSI, Thermo Fisher Scientific, Waltham, MA, USA) | Competitive | gp135 TM protein/A and B genotypes | Serum | 90.2% a/92.8% a 100.0% b/85.7% b |
qPCR | [27] |
| ID screen® MVV/CAEV indirect (IDvet Innovative Diagnostics, Grabels, France) | Indirect | peptides from the MVV/CAEV, gp135 and p25 proteins/A, B and E genotypes | Serum, plasma and milk | 100.0% a/97.8% a 91.7–100.0% b/97.6–98.9% b |
qPCR, ELISA A,B |
[27,64] |
| Eradikit™ SRLV screening test (IN3 diagnostic, Italia) | Indirect | gag and env peptides/A, B and E genotypes | Serum, plasma and milk | 96.1% a/99.4% a 100.0% b/94.6% b |
qPCR | [27] |
| Elitest MVV/CAEV (Hyphen BioMed, Neuville-sur-Oise, France) or Innotest MVV (Innogenetics, Gent, Belgium) | Indirect | MVV capsid rp25 and gp46 TM protein/EV-1 strain, A genotype | Serum | 98.0, 96.9, 97.8, 99.3% a/94.7, 99.2, 98.2, 99.4% a 95.8% b/99.7% b |
qPCR, Bayesian analysis, AGID and WB |
[27,32,46,65] |
| MVV/CAEV p28 Ab Screening Test (Idexx, Westbrook, ME, USA) | Indirect | peptide of TM protein (env gene) and of the recombinant p28 capsid protein/A genotype | Serum and plasma | 84.3% a/99.6% a 91.7% b/100.0% b |
qPCR | [27] |
| ELISA MAEDI VISNA/CAEV (Institut Pourquier, Montpellier, France) * |
Indirect | recombinant p28 gag protein and peptide of the env protein (gp135)/A genotype | Serum | 98% a/97.4% a | Bayesian analysis | [32] |
| CAEV/MVV Total Ab Test (Idexx, Westbrook, ME, USA) or Checkit CAEV/MVV (Dr. Bommeli AG, Bern, Switzerland) |
Indirect | Whole virus/strain OLV, A gentoype | Serum, plasma and milk | 98.6% b/99.3% b 91.4% c/98.9% c |
GAG-GST ELISA ** | [66] |
| Small Ruminant Lentivirus Antibody Test Kit, cELISA (VMRD, Pullman, WA, USA) |
Competitive | SU Antigen of gp135/B genotype | Serum | 98.6% a/96.9% a 100% b/96.4% b |
RIPA | [43,44] |
| INgezim Maedi screeningTM (Ingenasa, Eurofins Technologies, Spain) |
Indirect | synthetic peptides from the env protein/A and B genotypes | Serum | No published data | ||
| Enferplex Goat/Sheep Multi-Disease 5D (Enfer Scientific, Co. Kildare, Ireland) | Indirect | recombinant p25 core protein, TM1 gp46 synthetic peptide | Serum, plasma and milk | No published data |
Se: sensitivity; Sp: specificity; a Sensitivity and specificity values for sheep; b Sensitivity and specificity values for goats; c Sensitivity and specificity values for milk samples; gp: glycoprotein; TM: transmembrane; Ref: reference. * before merge of Institut Pourquier by Idexx Laboratories in 2007; ** recombinant GAG (group-specific antigens)-GST (glutathione S-transferase) fusion protein expressed in E. coli; A: Checkit CAEV/MVV monophasic Dr. Bommeli AG, Bern, Switzerland; B: ELISA MAEDI VISNA/CAEV Institut Pourquier, Montpellier, France.