FIG. 5.

Replication activity of chromosomally inactive ARS301 in the DpnI assay. (A) Schematic representation of the ARS301 plasmids used in the assay. The box indicates the DNA sequence derived from the yeast chromosome. The position of the ACS is indicated by the box containing the letter A. The shaded box and the box containing ABF1 indicate the binding site for Rap1p and Abf1p, respectively. (B) Replication activity of the ARS301 plasmid. The replication activities of the wild-type ARS1 plasmid and the ARS301 plasmid indicated in panel A were analyzed by the DpnI assay as described in Materials and Methods. The results obtained 48 h after transfection are indicated. The replication activities relative to that of the WTA plasmid were determined from two independent experiments and are indicated under each lane. +, present; −, absent.