TABLE 1.

Effect of GAL4 fusion proteins on the stability of ARS1 plasmids containing GAL4 binding sites

Expt no.	Activator	Reporter	Mitotic stability loss per generation (%) (± SD)
1		pYT528 (WTA)	5.2 ± 1.6
2		pYT530 (B3/Gal4)	6.1 ± 0.9
3		pYT530X3G (B3/Gal4X3)	6.1 ± 1.0
4		pYT530X5G (B3/Gal4X5)	6.2 ± 1.0
5	Gal4-VP16	pYT528	5.1 ± 1.1
6	Gal4-DBD	pYT530	6.1 ± 2.2
7	Gal4-VP16	pYT530	5.1 ± 1.1
8	Gal4	pYT530	4.2 ± 1.5
9	Gal4–c-Jun	pYT530	5.1 ± 1.5
10	Gal4-RAD	pYT530	5.3 ± 1.6
11	Gal4-DBD	pYT530XG3	5.4 ± 1.1
12	Gal4-VP16	pYT530XG3	7.2 ± 1.5
13	Gal4	pYT530XG3	6.3 ± 0.7
14	Gal4–c-Jun	pYT530XG3	6.7 ± 1.3
15	Gal4-RAD	pYT530XG3	7.4 ± 1.0
16	Gal4-DBD	pYT530XG5	5.3 ± 2.1
17	Gal4-VP16	pYT530XG5	8.1 ± 1.7
18	Gal4	pYT530XG5	10.6 ± 1.4
19	Gal4–c-Jun	pYT530XG5	7.4 ± 0.5
20	Gal4-RAD	pYT530XG5	8.0 ± 1.2