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. 2021 Sep 13;9:662868. doi: 10.3389/fcell.2021.662868

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Copyright © 2021 O’Reilly, Zeinabad, Nolan, Sefy, Williams, Tarunina, Hernandez, Choo and Szegezdi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bone marrow mesenchymal stromal cells (BMSCs), TGFβ-1 and all-trans retinoic acid (ATRA) can all reduce KG1a proliferation rate. (A) KG1a cells were labeled with 2.5 μM carboxyfluorescein succinimidyl ester (CFSE) and cultured alone, with alginate-encapsulated BMSCs, with TGFβ-1 (10 ng/ml), or with ATRA (2.5 μM) over an 8-day period. The graph shows the mean CFSE fluorescence intensity as a measure of CFSE retention in the live KG1a cells on days 0, 4, and 8 as determined by flow cytometry. (B) Viability measured with Annexin V on days 0, 4, and 8 by flow cytometry. ***p < 0.001 (Student’s t-test). MFI, mean fluorescence intensity.