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. 2021 Sep 13;9:662868. doi: 10.3389/fcell.2021.662868

FIGURE 3.

FIGURE 3

Hypoxia enhances the quiescence-inducing effect of bone marrow mesenchymal stromal cells (BMSCs) and TGFβ-1. KG1a cells were labeled with 2.5 μM carboxyfluorescein succinimidyl ester (CFSE) and cultured alone (KG1a) or with alginate-encapsulated BMSC and TGFβ-1 (10 ng/ml) over a 12-day period in normoxia or hypoxia (1% pO2). (A) CFSE retention measured in the live cell fraction on days 1, 8, and 12. (B) Viability measured with Annexin V on days 1, 8, and 12. (C) The absolute number of live cells per milliliter as determined by flow cytometry using CountBright beads on days 1, 8, and 12. (D) Cell cycle distribution based on Ki67 expression and DNA content (PI) on days 8 and 12. *p < 0.05; **p < 0.01; ***p < 0.001 (Student’s t-test).