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. 2021 Sep 13;12:740565. doi: 10.3389/fimmu.2021.740565

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Copyright © 2021 Tian, Zhao, Kang, Guo, Zhang, Shang, Zhang, Zhang, Jiang and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AS-IV remodeled the development of intestinal macrophages. (A) F4/80 protein expression in colon tissue was detected by immunofluorescence. Positive immunoreactivity for F4/80 protein expression is indicated by the red color. The slides were counterstained with DAPI (blue). In addition, the sum of OD was analyzed in (B). (C) Intestinal macrophages were collected and analyzed by fluorescence-activated cell sorting (FACS) after staining with anti-F4/80, anti-CD11b, and anti-MHCII (or anti-Ly6C). (D–H) The number of the different subpopulations in (C) was determined and quantitatively compared. One-way ANOVA test was used for statistical analyses. Bars represent means ± SD; ^* P < 0.05, ^**P < 0.01, ^*** P < 0.001. ns, no significance.