Figure 4.

AS-IV administration modulated the plasticity of intestinal macrophages. (A–H) Intestinal macrophages were collected with F4/80 maker by flow cytometric sorting. The relative mRNA expression levels of the inflammatory cytokines Il-1β, Il-12, Il-6, Tnf-α, CD206, Arg1, Il-10, and Tgf-β in F4/80 positive intestinal macrophages were determined by RT-PCR. The mean values ± SEMs are represented by bars. (I) The protein expression levels of the F4/80 (red color) and CD206 (green color) in colon tissue by immunofluorescence, and the slides were counterstained with DAPI (blue). (J) The number of F4/80⁺CD206⁺ cells in (I) were counted and quantitatively compared. One-way ANOVA test was used for statistical analyses. Bars represent means ± SD; ^* P < 0.05, ^**P < 0.01, ^*** P < 0.001. ns, no significance.