Figure 6.

AS-IV regulated macrophages phenotype through STAT1 signaling. (A–C) Western blotting analysis of STAT1, p-STAT1, STAT3, and p-STAT3 protein expression in mice colon tissue. (D–F) Western blotting analysis of STAT1, p-STAT1, STAT3, and p-STAT3 protein expression after BMDMs were stimulated with PBS (M^PBS), LPS + IFN-γ (M^LPS), or IL-4 (M^IL-4) following treatment with AS-IV. (G–I) Homology modeling and molecular docking were used to analyze the interaction between STAT1 and AS-IV. (J) HeLa cells were transiently transfected with STAT1 recognized reporters, TNF-α and AS-IV of different concentrations were added simultaneously. The luciferase activity was assessed 48 h later. (K–L) STAT1 was inhibited in M^PBS/M^LPS by STAT1 inhibitor, followed by AS-IV treatment, and then the expression of p-STAT1and STAT1were detected by western blotting analysis. (M–P) STAT1 was inhibited in M^PBS/M^LPS by STAT1 inhibitor, followed by AS-IV treatment, and then the expression of Il-1β, Tnf-α, CD206, and Tgf-β was detected by RT-PCR. student’s t-test or One-way ANOVA test was used for statistical analyses. Bars represent means ± SD; ^* P < 0.05, ^**P < 0.01, ^*** P < 0.001. ns, no significance.