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. 2021 Sep 13;12:731980. doi: 10.3389/fphar.2021.731980

FIGURE 3.

FIGURE 3

Effects of puerarin on SUMO2 expression and SUMOylation in mouse MI-RI model and hypoxia reoxygenation-challenged H9c2 cells. (A) In vivo effects of puerarin on SUMO2 expression and SUMOylation. After ischemia for 45 min and reperfusion for 24 h, heart tissues were collected and analyzed by Western blotting with antibodies against SUMO2, whereas GAPDH was analyzed as a control. (B) Quantification of SUMO2 expression and SUMOylation levels. The blots in panel A were quantified by a densitometric method. The results were shown as mean ± SD (n = 6). *p < 0.05. (C) In vitro effects of puerarin on SUMO2 expression and SUMOylation levels. After hypoxia (0.1% O2) for 3 h and reoxygenation for 24 h, H9c2 cells were collected and analyzed by Western blotting with antibodies against SUMO2, whereas GAPDH was analyzed as a control. (D) Quantification of SUMO2 expression and SUMOylation levels. The blots in panel C were quantified by a densitometric method. The results were presented as mean ± SD (n = 3). *p < 0.05.