FIGURE 5.

In silico binding of puerarin to ER and the activation of the ER pathway. (A) Molecular docking of puerarin to ER structure. Puerarin was docked to ER structure (PDB:1X7R) using software Autodock vina. (B) 2D plot illustrating the interactions of puerarin with amino acid residues of ER. Hydrogen bonds were shown in green lines. (C) Assay of ER-mediated gene transcription. Following transfection with 3xERE-TK-Luc and pRL-TK, H9c2 cells were treated with puerarin or estradiol and assayed for luciferase activity. The data were presented as mean ± SD (n = 3). *p < 0.05.