Figure S4. PTP1B re-introduction delays the return of the intracellular calcium level to the baseline.

Related to Fig 3. (A, B) Intracellular calcium measurements were performed using PTP1B KO cells with inducible expression of either empty vector control (GFP only) (A) or FLAG-PTP1B WT (B). Basal calcium levels were recorded for 30 s before the addition of 1 μg/ml anti-λ to stimulate BCR-induced calcium mobilization. Δ indicates the baseline corrected difference of the indo-1 (bound/unbound) ratio between the samples at 300 s. (C) Quantification of the calcium response in B cells at 300 s. Statistical analysis was performed using a t test (two-sided, n = 6, error bars = SEM).