Figure S1. Quantification of microtubule and centrin dynamics during the first three rounds of nuclear division.

(A) Durations of subsequent microtubule organization phases, that is, hemispindle, mitotic spindle, and extended spindle stage in dividing NF54 PfCentrin1-GFP parasites (n = 36 cells, three replicates) as shown in Fig 1C. Since most movies (32/36) were already started during hemispindle phase, we quantified the minimal mean length of hemispindle stage for the first division. To test for significant differences, we used Welch ANOVA–Games–Howell test. (B) Duration from start of the movie until appearance of clear first (n = 21, three replicates) and second (n = 19, three replicates) PfCentrin1-GFP signals.