Figure S5. Tagging strategy of endogenous Nup313 with 3xHA and glms tag using selection linked integration.

The 3′-end of the open reading frame of the PF3D7_1446500 gene was cloned into the pSLI-TGD-HA-glms construct to allow recombination with the endogenous locus inducing expression of the Neomycin selection cassette. PCRs using indicated primers (Table S3) show successful 5′ and 3′ integration into the genome and the absence of wild-type locus after secondary selection.