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. 2021 Sep 13;12:709135. doi: 10.3389/fphys.2021.709135

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Copyright © 2021 Chen, Zhou, Sun, Qu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The blocking effects of acute forskolin treatment on acute lactate injection induced inhibition of cAMP-PKA pathway. (A) Western blot analysis of cAMP-PKA pathway proteins after acute lactate and forskolin injections. (B) The ratios of P-PKA/PKA after acute lactate and forskolin injection. (C) The ratios of P-CREB/CREB after acute lactate and forskolin injection. AP and AD mean sacrificed after acute PBS and DMSO injection, respectively. ALF means sacrificed at 60min after acute lactate and forskolin injection (60min interval). AL-120: sacrificed 120min after acute lactate injection. AF-60: sacrificed 60min after acute forskolin injection. Three bands are used for statistics. The data are presented as the mean±SD, and significant differences among the groups were analyzed with one-way ANOVA. ^* p<0.05.