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. 2021 Sep 13;12:709135. doi: 10.3389/fphys.2021.709135

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Copyright © 2021 Chen, Zhou, Sun, Qu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analysis and relative fold protein expression of GPR81, P-CREB, CREB, and the ratios of P-PKA/PKA and P-CREB/CREB after chronic administration. (A) Western blot analysis of GPR81 and proteins involved in cAMP-PKA pathway. (B) Fold protein expression of GPR81. (C) The ratio of P-PKA/PKA. (D) Fold protein expression of P-CREB. (E) Fold protein expression of CREB. (F) The ratio of P-CREB/CREB. Three bands are used for statistics. The data are presented as the mean±SD, and significant differences among the groups were analyzed with one-way ANOVA. ^* p<0.05.