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. 2021 Sep 13;12:709135. doi: 10.3389/fphys.2021.709135

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Copyright © 2021 Chen, Zhou, Sun, Qu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The contents of lactic acid and TCA related metabolites in mitochondria after acute lactate injection and the colocalization of mitochondria and LDHA. (A) The relative abundance of Lactic acid, Succinic acid, Malic acid, and Oxalacetic acid in mitochondria. P: after acute PBS injection (n=3); L: after acute lactate injection (n=3). The data are presented as the mean±SD, and significant differences between the two groups were analyzed with the independent-samples t-test. ^* p<0.05. (B) Confocal laser scanning microscopic imaging of immunolabeled LDH (green), and HSP60 (red). Antibodies against HSP60 were used as a label of the mitochondria.