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. 2021 Sep 13;9:728057. doi: 10.3389/fcell.2021.728057

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Copyright © 2021 Tang, He, Huang, Bai, Wang, Wang, Yang, Ni, Hou, Wang, Zhou, Yao, Gong, Hou, Liu and Lan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hlf-labeled early cells contribute to long-term and multi-lineage adult hematopoiesis. (A) Representative FACS plots showing the proportions of Hlf-labeled cells in CD45⁺ cells and in each hematopoietic lineage in the peripheral blood from a 32-week old Hlf-CreER;ZsGreen mouse with tamoxifen administration at E9.5. (B) Graphs showing the dynamics of the labeling proportions in CD45⁺ leukocytes, granulocytes/monocytes (GMs), B lymphocytes, T lymphocytes, erythrocytes, and megakaryocytes, respectively, in the peripheral blood of Hlf-CreER;ZsGreen mice at 4–32 weeks old with tamoxifen administration at E9.5. Data are from three individuals represented by different colors. (C) Representative FACS plots showing the proportions of Hlf-labeled cells in LSK (Lin^–Sca-1⁺Kit⁺) cells, CMPs, GMPs, and MEPs in the bone marrow (BM) of 8–9 month-old Hlf-CreER;ZsGreen mice with tamoxifen administration at E9.5. (D) Graph showing the proportions of Hlf-labeled cells in LSK cells, CMPs, GMPs, MEPs, and CD45⁺ cells in the bone marrow (BM) of 8–9 month-old Hlf-CreER;ZsGreen mice with tamoxifen administration at E9.5. Data are shown as means ± SEM from three independent experiments represented by different colors. CMP, common myeloid progenitor; GMP, granulocyte-monocyte progenitor; MEP, megakaryocyte-erythroid progenitor. (E) Representative FACS plots (left) and graph (right) showing the proportions of Hlf-labeled cells in LT-HSCs, ST-HSCs, and MPPs in the bone marrow (BM) of adult Hlf-CreER;ZsGreen mice with tamoxifen administration at E9.5. LSKSLAM marker combination was used. Data are from three independent experiments represented by different colors. (F) Representative FACS plots (left) and graph (right) showing the proportions of Hlf-labeled cells in LT-HSCs, ST-HSCs, and MPPs in the bone marrow (BM) of adult Hlf-CreER;ZsGreen mice with tamoxifen administration at E9.5. LSKFlk2CD34 marker combination was used. Data are from three independent experiments represented by different colors. LT-HSC, long-term hematopoietic stem cell; ST-HSC, short-term hematopoietic stem cell; MPP, multipotent progenitor.