TABLE 3.
Regions of prohibitin involved in growth suppression and Raf-1 responsea
| Vector transfected | No. of colonies
|
|
|---|---|---|
| Expt 1 | Expt 2 | |
| pSVNeo | 165 | 175 |
| Raf-1–Neo, WT | 221 | 235 |
| Prohibitin-Neo, WT | 46 | 48 |
| Prohibitin 1-157 | 171 | 158 |
| Prohibitin 1-185 | 139 | 149 |
| Prohibitin 1-214 | 41 | 44 |
| Prohibitin 1-243 | 58 | 61 |
| Prohibitin Δ74-116 | 158 | 155 |
| Prohibitin Δ185-214 | 154 | 169 |
| Prohibitin+Raf-1 | 245 | 233 |
| Prohibitin 1-157+Raf-1 | 217 | 225 |
| Prohibitin 1-185+Raf-1 | 219 | 211 |
| Prohibitin 1-214+Raf-1 | 52 | 55 |
| Prohibitin 1-243+Raf-1 | 58 | 54 |
a
Approximately 10,000 T47D cells were transfected with 2 μg each of the indicated vectors. PCDNA3Raf-1 and pCDNA3 prohibitin vectors carried the neomycin resistance marker; colonies with 20 or more cells were counted after 14 days of selection in 40 μg of neomycin per ml. WT, wild type.