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The protein expression of NF-κB p65 was detected by Western blotting after treatment with Mg2+@GG/PAM hydrogel and LPS (A). The statistical analysis of p-IKBα, IKBα, and NF-κB p65 expression (means ± SD, n = 4). (B) RAW264.7 cells were stained with anti-p65 antibody and DAPI after culturing onto control, GG, GG/PAM, and Mg2+@GG/PAM hydrogel for 72 h and visualized by fluorescence microscopy. (C) *p < 0.05, **p < 0.01 vs control.
