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. 2021 Sep 13;12:721674. doi: 10.3389/fpls.2021.721674

FIGURE 1.

FIGURE 1

Transient silencing of WAT1 in tomato resulted in reduced susceptibility to Verticillium dahliae. (A) Phylogenetic tree based on amino acid sequences for WAT1 including potential ortholog from tomato (highlighted in gray square). Percentages indicate protein sequence similarity to AtWAT1 marked with an arrow. Number above node indicate branch support values. (B) Schematic overview of SlWAT1 (Solyc04g080940) with intron/exon locations as well as primers for cloning the VIGS fragment and for monitoring the SlWAT1 expression. (C) Expression levels (2–ΔΔCt) of plants treated with TRV::SlWAT1 in stems normalized to TRV::GUS 2 weeks after Agrobacterium tumefaciens treatment on a log10 scale. Data of two independent experiments with n ≥ 6 per experiment per genotype (t-test when compared with TRV::GUS with ∗∗∗p < 0.001). (D) Stunting (%) in Verticillium dahliae-inoculated plants when compared with the average of mock-inoculated plants at 21 dpi after transient silencing of SlWAT in eight independent experiments with n ≥ 8 per experiment per genotype (n ≥ 8, t-test when compared with TRV::GUS with ∗∗p < 0.01 and ∗∗∗p < 0.001).