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. 2021 Sep 13;11:722828. doi: 10.3389/fonc.2021.722828

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Copyright © 2021 Yu, Zhu, Cui, Du, Zhang, Ma and Guo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The effects of B4GALNT2 gene on the cell proliferation, invasion, migration and cycle of breast cancer cell lines were investigated by in-vitro cell experiments. (A) Using Celigo instrument, through counting for 5 consecutive days, it was found that the proliferation ability of the cells of the shB4GALNT2 group were significantly lower than that of the shCtrl group; (B) MTT assay was used for detection of the cell viability. After 5 consecutive days of detection, it was observed that the cell viability of shB4GALNT2 group was significantly lower than that of shCtrl group. (C) Flow cytometry was used for determining the early apoptosis rate of cells transfected with lentivirus, where it was found that the apoptosis rate of cells in shB4GALNT2 group was higher. (D, E) Transwell assay was used for measuring the migration and invasion abilities of cells after knockdown of B4GALNT2 gene, where it was found that the migration and invasion ability of cells in shB4GALNT2 group were significantly reduced. (F, G) Flow cytometry was used for detecting the cell cycle, where it was found that the B4GALNT2 gene knockdown resulted in significant G1 phase arrest. **p < 0.01, ***p < 0.001, ****p < 0.0001.