Figure 4.

Experiments on animals were conducted to verify the effect of B4GALNT2 gene knockdown of MDA-MB-231 breast cancer cell line on the proliferation of breast cancer cells in-vivo. (A) Model diagram of the mouse experiments. (B) Subcutaneous construction of tumor model in nude mice (each nude mouse was inoculated with 2×10⁶ tumor cells). (C) Nude mice tumor size; the black arrow indicates that there was no lump formation. (D) Tumor growth curves. (E) Statistical comparison of the weight of tumors in the two groups of nude mice. Note: NC=normal control; KD=knockdown of the B4GALNT2 gene. ***p < 0.001, ****p < 0.0001.