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. 2021 Sep 13;11:729799. doi: 10.3389/fonc.2021.729799

Figure 2.

Figure 2

Knocking down of FHL2 impairs Bzb-induced osteoblast differentiation of MM-MSCs. MM-MSCs were infected with shFHL2 and shSCR. (A) FHL2 protein levels were determined by Western blot. (B) Infected cells were cultured for 7 days with 2 nM Bzb with osteogenic induction medium (OBI). mRNA levels of FHL2 and osteoblast markers Runx2, ALP, and Col1A1 by quantitative RT-PCR. (C, D) Infected cells were cultured for 21 days with 2 nM Bzb with osteogenic induction medium (OBI) and 21 days. Alizarin red staining (C) was performed, and alkaline phosphatase index (API) (D) was determined. Results are expressed as mean ± SD (n = 3). ***p < 0.001 vs controls; N/S, not significant. Scale bars = 500 µm.