Skip to main content
. 2021 Sep 13;12:691279. doi: 10.3389/fphar.2021.691279

FIGURE 4.

FIGURE 4

MiR-9, regulated by HDAC6, suppresses AD. (A) Skin tissue lysates from each Nc/Nga mouse of each experimental group were subjected to miRNA array analysis. (B) MiR-9 mimic suppresses AD. ***, p < 0.001. Skin tissue lysates were subjected to qRT-PCR. Average values of three independent experiments were shown. (C) ELISA and qRT-PCR were performed. **, p < 0.01; ***, p < 0.001. Average values of three independent experiments were shown. (D) Skin tissue lysates (left) and skin mast cell lysates from skin tissue of Nc/Nga mouse of each experimental group (right) were subjected to immunoblot. Skin tissue lysates were subjected to immunoprecipitation (middle). Immunoprecipitation employing isotype-matched IgG antibody (2 μg/ml) was also performed. Representative blots of three independent experiments were shown. (E) H&E staining and toluidine blue staining were performed. Closed triangle represents degranulated mast cells. ***, p < 0.001.