Table 2.
The score value represents the percentage of homology between the spacer and its target (Biswas et al., 2013).
| Table 2A: Spacer BLAST scores of the main targeted bacterial species. | ||||||
|---|---|---|---|---|---|---|
| Genus | Protospacer species origin | % BLAST results | Average score | St. dev. | Min. | Max. |
| Clostridium | Clostridium botulinum | 28% | 28.76 | 4.97 | 21 | 38 |
| Staphylococcus | Staphylococcus aureus | 11% | 21.65 | 0.80 | 21 | 26 |
| Streptococcus | Streptococcus thermophilus | 7% | 24.38 | 1.16 | 22 | 26 |
| Escherichia | Escherichia coli | 4% | 22.25 | 0.54 | 22 | 24 |
| Borrelia | Borrelia miyamotoi | 2% | 22.19 | 0.68 | 21 | 23 |
| Bacillus | Bacillus thuringiensis | 2% | 23.84 | 1.95 | 21 | 28 |
| Clostridium | Clostridium perfringens | 2% | 23.38 | 2.88 | 21 | 32 |
| Lactococcus | Lactococcus lactis | 2% | 23.50 | 2.15 | 21 | 30 |
| Clostridium | Clostridium difficile | 1% | 23.00 | 0.82 | 22 | 24 |
| Staphylococcus | Staphylococcus epidermidis | 1% | 22.25 | 0.83 | 21 | 23 |
| Acinetobacter | Acinetobacter baumannii | 1% | 22.67 | 0.94 | 22 | 24 |
| Streptococcus | Streptococcus pyogenes | 1% | 23.27 | 2.21 | 21 | 28 |
| Bacillus | Bacillus cereus | 1% | 22.86 | 1.28 | 21 | 25 |
| Streptococcus | Streptococcus agalactiae | 1% | 23.87 | 0.88 | 22 | 26 |
| Streptococcus | Streptococcus mitis | 1% | 24.36 | 2.38 | 22 | 28 |
| Table 2B: Spacer BLAST scores of the main targeted C. botulinum mobile genetic elements (MGEs). | ||||||
| Mobile genetic element | Protospacer species origin | % BLAST results | Average score | St. dev. | Min. | Max. |
| p1_Cst | Clostridium botulinum | 6% | 31.36 | 4.98 | 21 | 38 |
| p4_BKT015925 | Clostridium botulinum | 5% | 29.42 | 4.43 | 21 | 38 |
| p6_Cst | Clostridium botulinum | 4% | 30.57 | 4.69 | 22 | 37 |
| CWou-2020a | Clostridium botulinum | 3% | 29.03 | 5.33 | 22 | 38 |
| p1_BKT015925 | Clostridium botulinum | 2% | 30.46 | 3.71 | 24 | 36 |
| p2_BKT015925 | Clostridium botulinum | 1% | 27.58 | 3.66 | 22 | 34 |
The higher the score, the better the homology, indicating a putative recent encounter. A: Clostridium botulinum is the main target of the spacers with up to 28% of the BLAST results, together with the highest average score. This illustrates the active dissemination of C. botulinum mobile genetic elements (MGEs) throughout Clostridium novyi sensu lato. B: The most encountered C. botulinum MGEs are p1_Cst>p4_BKT015925>p6_Cst, accounting for 15% of the BLAST results. Other C. botulinum MGEs (CWou-2020a, p1_BKT015925, and p2_BKT015925) account for 6% of the BLAST results, showing less frequent encounters. The average BLAST scores are roughly equivalent among the C. botulinum MGEs, suggesting concomitant dissemination.