Fig. 2. Intravital imaging of hepatic LDs accumulation induced by fasting. (A) Experimental scheme of intravital liver imaging after normal feeding for 12 hours or fasting for 12, 24, and 48 hours. (B) Quantitative analysis of the volumetric LD ratio in the liver. (C) Representative images of hepatic LDs in the liver of anesthetized mice after normal feeding or fasting. (D) Magnified images of individual LDs in hepatocytes (E) Average diameter of hepatic LDs. Data are presented as mean±standard deviation of mean. Scale bars, (B) 100 µm, (D) 20 µm.

LD, lipid droplet; SF44, Seoul-Flour 44.