Table 3. IC50 Obtained by the MTT Viability Test on HREC at Different Time Points for Compounds (+)-1 and (−)-1 and Their Precursors.
| cmpd | time (h) | IC50 (μM)a | pIC50 ± SEb |
|---|---|---|---|
| (±)-1c | 24 | 10.5 | 4.98 ± 0.02 |
| 48 | 11.1 | 4.95 ± 0.03 | |
| 72 | 10.1 | 4.99 ± 0.04 | |
| (+)-1 | 24 | 9.8 | 5.01 ± 0.03 |
| 48 | 6.4 | 5.19 ± 0.01 | |
| 72 | 4.4 | 5.35 ± 0.01 | |
| (−)-1 | 24 | 10.1 | 4.97 ± 0.07 |
| 48 | 9.0 | 5.04 ± 0.01 | |
| 72 | 6.8 | 5.16 ± 0.42 | |
| VPAc | 24 | 1217 | 2.91 ± 0.01 |
| 48 | 1449 | 2.84 ± 0.03 | |
| 72 | 1393 | 2.86 ± 0.01 | |
| (±)-HP-mIIc | 24 | >200d | |
| 48 | 128 | 3.89 ± 0.04 | |
| 72 | 69 | 4.16 ± 0.03 | |
| HPc | 24 | 3.7 | 5.43 ± 0.04 |
| 48 | 2.9 | 5.54 ± 0.08 | |
| 72 | 2.6 | 5.59 ± 0.07 |
a
IC50 values have been calculated with GraphPad Prism 5 for Windows using a nonlinear fit transform sigmoidal dose–response (variable slope). IC50 values are averaged from multiple determinations (n = 3, each of them).
b
pIC50 is defined as the −log(IC50).
c
Data taken from ref (34).
d
Cell viability reduction lower than 50% at 200 μM.