Table 1.

Primer pairs of myelination-related genes (Mbp, Plp1, Cspg4, Olig2), myelination factors expressed by microglia (Igf1 and Gpnmb) and housekeeping gene Rpl32 evaluated by RT-qPCR. Cspg4: chondroitin sulfate proteoglycan 4, Gpnmb: glycoprotein Nmb, Igf1: insulin-like growth factor 1, Mbp: myelin basic protein, Olig2: oligodendrocyte factor 2, Plp1: proteolipid protein 1, Rpl32: ribosomal protein L32.

Gene	5′primer	3′primer
Myelination-related genes
Mbp	CCC TCA CAG CGA TCC AAG TA	TAA AGA AGC GCC CGA TGG A
Plp1	GGC TCC AAC CTT CTG TCC AT	TCG GCC CAT GAG TTT AAG GA
Cspg4 (NG2)	AGA TCC TCC ACA ACA CAG GG	GGT ACC AGT GAC TCG GAA CA
Olig2	GCG CGA AAC TAC ATC CTC AT	CGT AGA TCT CGC TCA CCA GT
Myelination factor genes expressed by microglia
Igf1	GGC ATT GTG GAT GAG TGT TG	GTC TTG GGC ATG TCA GTG TG)
Gpnmb	AAA ACT GGG TCG GTG TTC AG	CAC CTT CGA GAT GGG AAT GT
Housekeeping gene
Rpl32	TTG TTG CTC CCA TAA CCG ATG	TTA AGC GAA ACT GGC GGA AAC