Table 2.
Characterization of myelinated axons in the rostrum of the corpus callosum of P30 offspring exposed to mHFD. 10–15 images/animal, n = 4 animals/group (density); n = 302–436 axons/group, N = 4 animals/group (g-ratio, cytosolic channels, decompacted myelin and shape descriptors). Statistical significance was determined by 2-way ANOVA followed by Bonferroni post-hoc analysis, where ∗ p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. ♂: male, ♀: female, a.u.: arbitrary unit, CD: control diet, mHFD: maternal high-fat diet.
| Parameters | Mean ± standard error of the mean |
F | p | ||||
|---|---|---|---|---|---|---|---|
| Male |
Female |
||||||
| CD | mHFD | CD | mHFD | ||||
| Density (axons/μm2) | 0.196 ± 0.014 | 0.205 ± 0.019 | 0.195 ± 0.024 | 0.203 ± 0.010 | Sex∗Diet: 0.0001575 Sex: 0.009132 Diet: 0.2451 |
Sex∗Diet: 0.9902 Sex: 0.9254 Diet: 0.6295 |
|
| G-ratio (a.u.) | 0.775 ± 0.004 | 0.780 ± 0.004 | 0.786 ± 0.005 | 0.776 ± 0.005 | Sex∗Diet: 2.878 Sex: 0.8895 Diet: 0.3245 |
Sex∗Diet: 0.0900 Sex: 0.3458 Diet: 0.5690 |
|
| Cytosolic channels | Ratio (a.u.) | 0.563 ± 0.025 | 0.413 ± 0.024 | 0.464 ± 0.029 | 0.389 ± 0.025 | Sex∗Diet: 2.165 Sex: 5.825 Diet: 19.33 |
Sex∗Diet: 0.1414 Sex: 0.0159∗ Diet: < 0.0001∗∗∗∗ |
| Area (μm2) | 0.0222 ± 0.002 | 0.0123 ± 0.001 | 0.0183 ± 0.002 | 0.0177 ± 0.002 | Sex∗Diet: 5.293 Sex: 0.1385 Diet: 6.532 |
Sex∗Diet: 0.0215∗ Sex: 0.7098 Diet: 0.0107∗ |
|
| Ratio decompacted myelin (a.u.) | 0.341 ± 0.024 | 0.360 ± 0.023 | 0.358 ± 0.028 | 0.357 ± 0.025 | Sex∗Diet: 0.1689 Sex: 0.07284 Diet: 0.1367 |
Sex∗Diet: 0.6812 Sex: 0.7873 Diet: 0.711 |
|
| Shape descriptors of myelinated axons | Circularity (a.u.) | 0.809 ± 0.005 | 0.783 ± 0.004 | 0.771 ± 0.006 | 0.762 ± 0.005 | Sex∗Diet: 2.365 Sex: 32.27 Diet: 11.68 |
Sex∗Diet: 0.1243 Sex: < 0.0001∗∗∗∗ Diet: 0.0006∗∗∗ |
| Solidity (a.u.) | 0.977 ± 0.008 | 0.974 ± 0.001 | 0.975 ± 0.001 | 0.975 ± 0.001 | Sex∗Diet: 3.077 Sex: 0.4825 Diet: 1.518 |
Sex∗Diet: 0.0796 Sex: 0.4874 Diet: 0.2181 |
|
| Roundness (a.u.) | 0.682 ± 0.001 | 0.611 ± 0.007 | 0.608 ± 0.010 | 0.565 ± 0.007 | Sex∗Diet: 3.311 Sex: 56.28 Diet: 50.52 |
Sex∗Diet: 0.1266 Sex: < 0.0001∗∗∗∗ Diet: < 0.0001∗∗∗∗ |
|
| Aspect ratio (a.u.) | 1.586 ± 0.027 | 1.737 ± 0.023 | 0.786 ± 0.036 | 1.895 ± 0.027 | Sex∗Diet: 399.9 Sex: 179.7 Diet: 690.9 |
Sex∗Diet: < 0.0001∗∗∗∗ Sex: < 0.0001∗∗∗∗ Diet: < 0.0001∗∗∗∗ |
|