Table 3.

mHFD effects on oligodendrocytes population density, distribution and maturation, as well as fluorescence intensity of Olig2 and Nogo-A within the rostrum of the corpus callosum of P30 offspring. 3–4 sections/animal, n ​= ​5 animals/group. Statistical significance was determined by 2-way ANOVA, where ∗ p ​< ​0.05. ♂: male, ♀: female, a.u.: arbitrary unit, CD: control diet, mHFD: maternal high-fat diet.

Parameters		Mean ​± ​standard error of the mean				F	p
		Male		Female
		CD	mHFD	CD	mHFD
Density (cells/mm2)	Olig2+	1085 ​± ​199	905.0 ​± ​44.8	1256 ​± ​260.7	1099 ​± ​203.9	Sex∗Diet: 0.003761 Sex: 0.9492 Diet: 0.8141	Sex∗Diet: 0.9519 Sex: 0.3454 Diet: 0.3812
	Olig2+/Nogo-A-	1085 ​± ​199	905.0 ​± ​44.8	1256 ​± ​260.7	1099 ​± ​203.9	Sex∗Diet: 0.003761 Sex: 0.9492 Diet: 0.8141	Sex∗Diet: 0.9519 Sex: 0.3454 Diet: 0.3812
	Olig2+/Nogo-A+	189.1 ​± ​42.5	152.0 ​± ​15.4	243.7 ​± ​80.5	174.8 ​± ​38.9	Sex∗Diet: 0.1202 Sex: 0.7094 Diet: 1.331	Sex∗Diet: 0.7336 Sex: 0.4129 Diet: 0.2666
Spacing index (a.u.)	Olig2+	0.356 ​± ​0.018	0.339 ​± ​0.003	0.341 ​± ​0.017	0.343 ​± ​0.009	Sex∗Diet: 0.4879 Sex: 0.1592 Diet: 0.3077	Sex∗Diet: 0.4949 Sex: 0.6952 Diet: 0.5868
	Olig2+/Nogo-A+	0.175 ​± ​0.030	0.158 ​± ​0.42	0.156 ​± ​0.024	0.151 ​± ​0.022	Sex∗Diet: 0.04033 Sex: 0.1857 Diet: 0.1264	Sex∗Diet: 0.8434 Sex: 0.6723 Diet: 0.7268
Integrated density (a.u.)	Olig2+	46454 ​± ​4443	37809 ​± ​3116	45577 ​± ​3186	36844 ​± ​2990	Sex∗Diet: 0.0001597 Sex: 0.06978 Diet: 6.220	Sex∗Diet: 0.9901 Sex: 0.7950 Diet: 0.0240∗
	Nogo-A+	57635 ​± ​5100	61180 ​± ​3859	69984 ​± ​2259	62973 ​± ​1497	Sex∗Diet: 2.310 Sex: 4.145 Diet: 0.2489	Sex∗Diet: 0.1481 Sex: 0.0587 Diet: 0.6246
Maturation	% cell density	17.45 ​± ​1.37	16.96 ​± ​1.78	18.36 ​± ​2.60	15.93 ​± ​1.36	Sex∗Diet: 0.3038 Sex: 0.001173 Diet: 0.6846	Sex∗Diet: 0.5896 Sex: 0.9731 Diet: 0.4210
	Nogo-A+/Olig2+ integrated density (a.u.)	1.000 ​± ​0.101	1.086 ​± ​0.071	0.816 ​± ​0.074	1.070 ​± ​0.040	Sex∗Diet: 1.251 Sex: 1.803 Diet: 5.175	Sex∗Diet: 0.2798 Sex: 0.1981 Diet: 0.0370∗