Table 4.

mHFD effects on microglial ultrastructure in the rostrum of the corpus callosum of P30 offspring. n ​= ​33–41 microglia/group, N ​= ​4 animals/group. Statistical significance was assessed by mixed effect model, where ∗ p ​< ​0.05, ∗∗p ​< ​0.01. #: number, CD: control diet, ER/Golgi: endoplasmic reticulum and Golgi apparatus, mHFD: maternal high-fat diet.

Parameters			Mean ​± ​standard error of the mean				F	p
			Male		Female
			CD	mHFD	CD	mHFD
Organelles	# Lysosomes	Primary	1.727 ​± ​0.434	2.000 ​± ​0.447	1.892 ​± ​0.487	2.200 ​± ​0.476	Sex∗Diet: 0.003469 Sex: 0.1699 Diet: 0.4150	Sex∗Diet: 0.9531 Sex: 0.6808 Diet: 0.5205
		Secondary	0.273 ​± ​0.090	0.359 ​± ​0.101	0.459 ​± ​0.138	0.390 ​± ​0.120	Sex∗Diet: 0.4536 Sex: 0.8919 Diet: 0.005444	Sex∗Diet: 0.5017 Sex: 0.3465 Diet: 0.9413
		Tertiary	0.152 ​± ​0.077	0.000 ​± ​0.000	0.162 ​± ​0.073	0.073 ​± ​0.041	Sex∗Diet: 0.3312 Sex: 0.5953 Diet: 4.901	Sex∗Diet: 0.5658 Sex: 0.4416 Diet: 0.0284∗
	# Lipidic inclusion		0.242 ​± ​0.107	0.128 ​± ​0.066	0.162 ​± ​0.073	0.122 ​± ​0.062	Sex∗Diet: 0.2340 Sex: 0.3197 Diet: 1.019	Sex∗Diet: 0.6293 Sex: 0.5726 Diet: 0.3145
	# Endosomes	Empty	0.182 ​± ​0.068	0.128 ​± ​0.066	0.189 ​± ​0.076	0.146 ​± ​0.066	Sex∗Diet: 0.006046 Sex: 0.03394 Diet: 0.4854	Sex∗Diet: 0.9381 Sex: 0.8541 Diet: 0.4871
		Content	0.455 ​± ​0.116	0.513 ​± ​0.151	0.378 ​± ​0.131	0.439 ​± ​0.171	Sex∗Diet: 6.439 $\times$10-5 Sex: 0.2576 Diet: 0.1620	Sex∗Diet: 0.9936 Sex: 0.6125 Diet: 0.6879
	# Dilated ER/golgi cisternae		6.939 ​± ​1.021	8.256 ​± ​1.190	7.189 ​± ​1.789	8.098 ​± ​1.906	Sex∗Diet: 0.01696 Sex: 0.0008399 Diet: 0.5029	Sex∗Diet: 0.8966 Sex: 0.9769 Diet: 0.4794
	# Mitochondria	Elongated	0.394 ​± ​0.150	0.462 ​± ​0.137	0.324 ​± ​0.140	0.732 ​± ​0.229	Sex∗Diet: 0.9589 Sex: 0.3341 Diet: 1.874	Sex∗Diet: 0.3291 Sex: 0.5642 Diet: 0.1731
		Total	2.697 ​± ​0.373	2.718 ​± ​0.442	3.162 ​± ​0.763	2.951 ​± ​0.549	Sex∗Diet: 0.04288 Sex: 0.3889 Diet: 0.02877	Sex∗Diet: 0.8362 Sex: 0.5338 Diet: 0.8655
Interactions with their microenvironment	# Synaptic elements	Pre	1.303 ​± ​0.273	2.821 ​± ​0.526	0.865 ​± ​0.202	1.634 ​± ​0.320	Sex∗Diet: 1.071 Sex: 5.051 Diet: 10.01	Sex∗Diet: 0.3023 Sex: 0.0261∗ Diet: 0.0019∗∗
		Post	0.576 ​± ​0.157	1.282 ​± ​0.257	0.432 ​± ​0.192	0.659 ​± ​0.173	Sex∗Diet: 1.411 Sex: 3.598 Diet: 5.319	Sex∗Diet: 0.2368 Sex: 0.0598 Diet: 0.0225∗
	# Myelinated axon		3.000 ​± ​0.538	3.615 ​± ​0.478	3.054 ​± ​0.586	3.927 ​± ​0.425	Sex∗Diet: 0.06436 Sex: 0.1298 Diet: 2.151	Sex∗Diet: 0.8001 Sex: 0.7192 Diet: 0.1446
	# Degraded myelin		1.000 ​± ​0.199	0.923 ​± ​0.215	0.811 ​± ​0.204	1.341 ​± ​0.272	Sex∗Diet: 1.745 Sex: 0.2483 Diet: 0.9732	Sex∗Diet: 0.1886 Sex: 0.6190 Diet: 0.3255
	# Contacts with brain cells	Astrocyte	0.061 ​± ​0.042	0.154 ​± ​0.059	0.243 ​± ​0.081	0.049 ​± ​0.034	Sex∗Diet: 6.371 Sex: 0.4631 Diet: 0.7886	Sex∗Diet: 0.0127∗ Sex: 0.4972 Diet: 0.3760
		Neuron	0.061 ​± ​0.042	0.051 ​± ​0.036	0.135 ​± ​0.069	0.024 ​± ​0.024	Sex∗Diet: 1.263 Sex: 0.2786 Diet: 1.770	Sex∗Diet: 0.2629 Sex: 0.5984 Diet: 0.1855
		Oligodendrocyte	0.061 ​± ​0.042	0.00.26 ​± ​0.026	0.216 ​± ​0.088	0.098 ​± ​0.047	Sex∗Diet: 0.5692 Sex: 4.207 Diet: 1.918	Sex∗Diet: 0.4518 Sex: 0.0420∗ Diet: 0.1682
		Oligodendrocyte progenitor	0.000 ​± ​0.000	0.026 ​± ​0.026	0.054 ​± ​0.038	0.024 ​± ​0.024	Sex∗Diet: 1.083 Sex: 0.9869 Diet: 0.005728	Sex∗Diet: 0.2998 Sex: 0.3221 Diet: 0.9398
		Blood vessel	0.091 ​± ​0.051	0.077 ​± ​0.043	0.027 ​± ​0.027	0.049 ​± ​0.034	Sex∗Diet: 0.2075 Sex: 1.376 Diet: 0.009800	Sex∗Diet: 0.6494 Sex: 0.2428 Diet: 0.9213
	# Extracellular space pocket		4.182 ​± ​0.512	3.590 ​± ​0.575	3.216 ​± ​0.384	3.610 ​± ​0.511	Sex∗Diet: 0.9501 Sex: 0.8745 Diet: 0.03855	Sex∗Diet: 0.3313 Sex: 0.3512 Diet: 0.8446
	# Extracellular digestion		0.455 ​± ​0.145	0.308 ​± ​0.098	0.216 ​± ​0.088	0.293 ​± ​0.112	Sex∗Diet: 1.009 Sex: 1.298 Diet: 0.1002	Sex∗Diet: 0.3169 Sex: 0.2565 Diet: 0.7521