Fig. 1.

Anxiety-related behaviors of CD300f−/− male mice in different behavioral contexts. The total distance traveled in the OF was measured (meters) to evaluate mice spontaneous ambulatory activity (n ​= ​15 WT and 18 CD300f−/−, 1.A), and the % of time spent in the center of the OF was used to evaluate anxious-like behavior (n ​= ​14 WT and 18 CD300f−/−, 1.B). The total rearing (n ​= ​6 WT and 7 CD300f−/−, 1.C) and the number of unsupported (n ​= ​6 WT and 7 CD300f−/−, 1.D) rearing were counted to evaluate explorative behavior and emotionality. Grooming behavior was recorded (seconds) to evaluate mice habituation to a new environment (n ​= ​6 WT and 7 CD300f−/−, 1.E). The % of time spent in the open arms of the EPM (calculated using the total time in the open arms ​+ ​the total time in the closed arms as 100%) was indicative of anxiety-like behavior (n ​= ​9 WT and 8 CD300f−/−, 1.F). Heatmaps illustrating WT and CD300f−/− mice behavior in the EPM, respectively (1.G). The number of entries in the closed arms of the EPM was indicative of spontaneous locomotion (n ​= ​9 WT and 8 CD300f−/−, 1.H). The latency to start eating in the NSF test was used as an indicative of anxious-like behavior in a new environment (n ​= ​12 WT and 17 CD300f−/−, 1.I). Data are represented as mean ​± ​S.E.M. ∗ represents p ​< ​0.05, and ∗∗ represents p ​< ​0.01 compared to WT. Abbreviations: OF: Open Field; EPM: Elevated Plus Maze; NSF: Novelty Suppressed Feeding; WT: Wild-type mouse; CD300f−/−: CD300f knockout mouse.