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. 2020 Apr 13;4:100069. doi: 10.1016/j.bbih.2020.100069

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Serum autoAbs to subcellular protein fractions of brain tissue. Subcellular protein fractions from whole BTBR (B6) and C57BL/6 (B6) brains were used to detect brain-reactive IgG antibodies from BTBR mice by ELISA. ELISA plates were coated with 10 μg of each protein fraction per well and 100 μl of BTBR sera (n = 3, mean ± SD) diluted 1/100 were assayed as described. The ∗ indicates a significant difference between BTBR and B6 brain protein fractions.