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. 2020 Apr 13;4:100069. doi: 10.1016/j.bbih.2020.100069

Fig. 4.

Fig. 4

Serum autoAbs to subcellular protein fractions of neuronal cell line and human brain regions. Western blot analyses of BTBR and B6 IgG specificities. An equal amount of protein from CATH.a (L1), MN9D (L2), NE-GFP-4C (L3) and N1E-115 (L4) cell homogenates were separated with 10–12% SDS-PAGE gel and blotted with BTBR (A) or B6 (D) sera as primary antibodies. This experiment was done more than three times. For (B and E), equivalent amounts of protein from BTBR brain subcellular fractions (Nuc-s,L1; Mito-s, L2; Mito-M; L-3) and B6 brain sub-cellular fractions (Nuc-s, L4; Mito-s, L5; Mito-m, L6) were loaded, separated by 10–12% SDS-PAGE, and probed with diluted BTBR (B) or B6 (E) sera as primary antibody. This experiment was done more than six times. For C and F, equivalent amounts of protein from different human brain sections (frontal cortex-L1, Parietal cortex-L2, Hippocampus-L3 and cerebellum-L4) were loaded, separated by 10–12% SDS-PAGE, and blotted with diluted BTBR (C) or B6 (F) sera as a primary antibody. Data are representative of minimum three independent experiments with equivalent results.