Fig. 5.

Serum autoAbs to ENO1 and DLST ELISA of BTBR and B6 serum Abs to Eno1 (A) and Dlst (B). A 96-well plate was coated with 1 ​μg rabbit polyclonal Ab to ENO1 or DLST, blocked and used to capture Ag from 10 ​μg SCID brain homogenate for assessment of anti-ENO1 or anti-DLST IgG Ab in BTBR or B6 sera, which was diluted 1:10, and detected with HRP conjugated goat ant-mouse IgG Fc. The ∗ indicates a significant difference between BTBR and B6 Ab levels. ∗ ​= ​p ​< ​0.05, ∗∗ ​= ​p ​< ​0.01, ∗∗∗ ​= ​p ​< ​0.001.