Fig. 7.

CD4⁺ and CD8⁺ T cells in BTBR and B6 mice. BTBR mice have significantly more CD4⁺ and CD8⁺ T cells than B6 mice. Representative flow cytometric analyses are shown for CD4⁺ T cells and CD8⁺ T cells in the blood (A) and spleen (D) of B6 and BTBR mice. To observe the CD4⁺ and CD8⁺ populations the cells were first gated on the CD3⁺ population; B cells were gated out based on CD19⁺ cells. Frequencies and numbers of CD3⁺CD8⁺ T cells and CD3⁺CD4⁺ cells and populations in the blood (B &C) and spleens (E&F) of B6 and BTBR mice are shown. Data are representative of three or four independent experiments with 4 pairs of mice in each experiment. P values determined by unpaired two-tailed Student’s t-test are indicated and p ​< ​0.05 is considered as significantly different. Error bars indicate mean ​± ​SEM. ∗ indicates a significant difference between the two strains. ∗ ​= ​p ​< ​0.05, ∗∗ ​= ​p ​< ​0.01, ∗∗∗ ​= ​p ​< ​0.001.