Fig. 6.
Effects of an i. c.v injection of PGE2 on the abdominal core temperature and locomotor activity of WT and TRPM8 KO mice. A: Schematic diagram showing time schedule of the experiment; mice received an i.c.v. injection of 16 or 160 nmol/kg PGE2 and body temperature and locomotor activity were measured with a G2 E-mitter transponder at an ambient temperature of 25 ± 0.5 °C. B: The i. c.v. injection of PGE2 induced prominent fever in both WT and TRPM8 KO mice. C: The temperature index (ΔoC × hr) as significantly increased in WT and TRPM8 KO mice by the i.c.v. injection of PGE2. D: Cumulative locomotor activity was not significantly different among treatment groups. Data (WT: saline, n = 6; 16 nmol/kg PGE2, n = 4; 160 nmol/kg PGE2, n = 4; TRPM8 KO: saline, n = 7; 16 nmol/kg PGE2, n = 5; 160 nmol/kg PGE2) are expressed as the mean (±s.e.m.). Statistically significant difference: lines in B indicate a significant period (p < 0.05). ∗p < 0.05, ∗∗p < 0.01 among treatment groups by a one-way ANOVA with Tukey's post hoc test.