Fig. 1.

Schematic drawing of the experimental procedures and VBC/PBM administration schedule. In A experimental design for rats underwent treatment with VBC alone; in B experimental design for rats underwent treatment with PBM alone and C experimental design for rats underwent treatment with VBC ​+ ​PBM. Rats were allowed to acclimate to the test chamber for at least 3 days before the first measurement (baseline – BL). Afterwards, was performed the CCI-IoN surgery, and for group of animals treated with vitamins cocktail (VBC and VBC ​+ ​PBM) was also injected the first administration of VBC cocktail; the animals belonging to the group PBM or VBC ​+ ​PBM beginning the PBM application 3 days after CCI-IoN and follow every other day; the evaluation of the nociceptive threshold by von Frey test was tested in every other day from day 4 until day 22. All animals were tested during the light cycle at about the same time of the day (8:00 a.m.–12:00 a.m.).