Table 1.

Testing Sites, Platforms Used, and Study Phase Participation

Laboratory #	Platform Used	Assays	Phase 1	Phase 2	cfDNA Extraction Kit Used
1	Droplet digital PCR	Bio-Rad EGFR assay and IDT (Integrated DNA Techonologies) designed primers and probes	X	X	QIAamp circulating nucleic acid kit
2	NGS (S5)	ThermoFisher Oncomine Lung cfDNA assay	X	X	QIAamp circulating nucleic acid kit
3	NGS	IlluminaTruSight Tumor 15 - TST15 panel	X	X	QIAamp circulating nucleic acid kit
4	Real-time PCR (Cobas 4800)	Cobas EGFR mutation test v2	X	X	Cobas cfDNA sample preparation kit
5	Real-time PCR	Entrogen ctEGFR mutation detection kit	X	X	Nucleosnap kit for plasma DNA
6	Real-time PCR	Entrogen ctEGFR mutation detection kit	X	X	QIAamp circulating nucleic acid kit
7	Real-time PCR (Cobas 4800)	Cobas EGFR Mutation test v2	X	X	Cobas cfDNA sample preparation kit
8	Real-time PCR (Cobas 4800)	Cobas EGFR mutation test v2	X		Cobas cfDNA sample preparation kit
9	Mass array	UltraSEEK lung panel	X		QIAamp circulating nucleic acid kit
Reference laboratory	Droplet digital PCR	Bio-Rad EGFR assay and IDT designed primers and probes	X (1b only)	X	QIAamp circulating nucleic acid kit

#, number; cfDNA, cell-free DNA; IDT, integrated DNA Technologies; NGS, next-generation sequencing; PCR, polymerase chain reaction.