Fig. 4.

Pomegranate extract and urolithin-A do not induce cell death. 3-weeks differentiated neural cells were infected with Pru T. gondii at MOI 1 for 24 ​h followed by treatment with vehicle control DMSO, 300 ​μg/ml PE, 50 ​μM or 100 ​μM UA for 24 ​h. (a) Neurite outgrowth staining kit to assess cell viability. Fluorescence images (top) and quantification (bottom) of green (viable cells) to red (total cells) signal ratio (n ​= ​4). (b) MTS assay to quantify cell viability. The graphs were plotted as fold change against DMSO control (n ​= ​5). All values are mean ​± ​S.E.M. One-way ANOVA with Dunnett’s multiple comparisons test (DMSO set as the control) was performed. Scale bar: 100 ​μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)