Fig. 5.
Urolithin-A reduces brain cystsize and alters the innate response of infected mice towards cat odor. (a) Timepoints for the in-vivo workflow. (b) 8 representative images of brain cysts observed in infected mice administered with 30 μg UA or equivalent volume of DMSO. (c) Quantification of cyst count per gram of brain homogenate tabulated from 4 DMSO and 6 UA mice. The line inside the box represents the median and the cross (x) indicates the average. (d) Analysis of the diameter of cysts in the brains of infected animals. A total of 175 and 77 cysts were analyzed, tabulated from 4 DMSO and 6 UA mice, respectively. Each dot represents one cyst. The horizontal line represents the average diameter of the cysts. (e) Bobcat urine aversion assay behavioral paradigm. (f–g) Time spent in the left bisect (left) and the aversion index (right) during the pre-exposure and exposure phase. The aversion index is tabulated as timeleft/timeright. The line inside the box represents the median and the cross (x) indicates the average. All values are mean ± S.E.M. Unpaired two-tailed t-test with a 95% confidence interval was performed. Differences against DMSO control are significant for ∗p ≤ 0.05 and ∗∗p ≤ 0.001. Scale bar: 20 μm.