. 1999 May;37(5):1260–1264. doi: 10.1128/jcm.37.5.1260-1264.1999

TABLE 2.

Efficacy of DNase treatment of cellular RNA extracted from cellular specimens

Step	No. of HIV-1 RNA copies/PCR mixture for the following specimens with the indicated no. of DNA copies^a:
	Amplicor RNA diluent		Control, 20,000 DNA copies^b ^c	Patient A^d		Patient B, no DNA^d	Patient C, no DNA^d
	200 DNA copies^e	20,000 DNA copies^e	Control, 20,000 DNA copies^b ^c	200 DNA copies^c	No DNA	Patient B, no DNA^d	Patient C, no DNA^d
1. No processing	206	42,730	ND^f	ND	ND	ND	ND
2. RNeasy protocol without DNase I	ND	ND	70,503	ND	ND	ND	ND
3. Standard protocol with 1× DNase I	ND	ND	9	49	65	13	5
4. Extended protocol with 2× DNase I	ND	ND	<2	51	66	9	6
No. of samples^g	1	1	2	2	2	1	1

Copies of restriction enzyme (PvuII)-digested diluted plasmid pBH10 (5); calculation included the consideration that one molecule of double-stranded DNA represents two templates for the PCR.

PBMCs from an HIV-negative donor.

DNA was added after step 2.

PBMCs from HIV-positive donors receiving HAART.

DNA was added after step 1.

ND, not determined.