Fig. 5.

B. longum supplementation does not alter the faecal microbiome during exam stress.

Following 16S compositional sequencing of faecal samples from timepoints before and after each visit (Fig. 1A, Table 2) was performed. Species diversity was not changed significantly at any visit compared within or between placebo and probiotic groups as measured by Chao1 (A), Simpson Index (B) Shannon Index (C) PD Whole Tree (D) and Observed Species (E). Relative abundance at the phylum (F), Family (G) and Genus (H) level there was no significant difference in the percentage of taxa in each group before or during the exam stress between participants receiving placebo or probiotic. Fig. 5 A-E, graphs of alpha-diversity represented by box-whisker plots with data represented as median with inter-quartile range and min/max values as error bars, n ​= ​8 in Bif. longum and n ​= ​12 in placebo group.