Fig. 2.

HSP70 treatment improves behavior impairments induced by 6-OHDA. On day 18, the ladder rung walking test was performed. A qualitative evaluation was performed for each step using a foot fault scoring system, using a 7-category scale: (0) total miss, (1) deep slip, (2) slight slip, (3) replacement, (4) correction, (5) partial placement and (6) correct placement. [A] The average of scores per animal was measured. [B] The percentual of errors was also measured, counting scores of 0, 1 and, 2 as errors. On day 19, the open field test was performed. [C] The total distance traveled (m); [D] average speed (m/s); [E] total time immobile (s); [F] total time mobile (s); [G] total time freezing (s); and [H] total time in the central zone, corners zone and sides zones (s) were determined in a 5 ​min session, using the video tracking system ANY-maze. On day 20, the apomorphine-induced rotation test was performed, and [I] 360-degree contralateral rotations were quantified in a 30 ​min session. Values represent mean ​± ​SD. Two-way analysis of variance and Tukey’s Multiple Comparison posthoc test was applied to all data. The p values are represented as followed: ∗∗∗p ​< ​0.001, ∗∗p ​< ​0.01, ∗p ​< ​0.05.