Fig. 3.

HSP70 treatment reduces 6-OHDA-induced dopaminergic denervation in SNpc. [A] Representative immunofluorescence images of SNpc co-immunostained for TH (green) and DAPI (blue). Images from ipsilateral sides of are Control (vehicle ​+ ​saline), HSP70 (vehicle ​+ ​HSP70), 6-OHDA (6-OHDA ​+ ​saline), and 6-OHDA ​+ ​HSP70 groups are shown. Guide bars represent 400 ​μm. Images are representative of independent experiments from eight animals per group. [B] The quantification of TH content was obtained using the software ImageJ measuring the pixels of immunofluorescence images with 10X of magnification, and results were expressed as the ratio of TH staining in ipsilateral side per contralateral side. Values represent mean ​± ​SD. Two-way analysis of variance and Tukey’s Multiple Comparison posthoc test was applied to all data. The p values are represented as followed: ∗∗p ​< ​0.01, ∗p ​< ​0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)