Fig. 4.

HSP70 treatment reduces 6-OHDA-induced glial activation in SNpc. [A] Representative immunofluorescence images of SNpc co-immunostained for Iba1 (green), GFAP (red), and DAPI (blue). Images from ipsilateral sides of Control (vehicle ​+ ​saline), HSP70 (vehicle ​+ ​HSP70), 6-OHDA (6-OHDA ​+ ​saline), and 6-OHDA ​+ ​HSP70 groups are shown. Merge images were obtained with 20X and 40X of magnification, guide bars represent 200 ​μm and 100 ​μm. Details of microglia and astrocytes morphology are shown in separated canals. Images are representative of independent experiments from eight animals per group. The quantification of Iba1 [B] and GFAP [C] content was obtained using the software ImageJ measuring the pixels from the ipsilateral SNpc region of immunofluorescence images with 4X of magnification, and results were expressed as a percentage to control. Values represent mean ​± ​SD. Two-way analysis of variance and Tukey’s Multiple Comparison posthoc test was applied to all data. The p values are represented as followed: ∗∗p ​< ​0.01, ∗p ​< ​0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)