Fig. 6.

HSP70 treatment increases HSP70 protein content in the SNpc of rats induced by 6-OHDA. [A] Representative immunofluorescence images of SNpc co-immunostained for HSP70 (red) and DAPI (blue). Images from ipsilateral sides of are Control (vehicle ​+ ​saline), HSP70 (vehicle ​+ ​HSP70), 6-OHDA (6-OHDA ​+ ​saline), and 6-OHDA ​+ ​HSP70 groups are shown. Guide bars represent 400 ​μm. Images are representative of independent experiments from eight animals per group. [B] The quantification of HSP70 content was obtained using the software ImageJ measuring the pixels of immunofluorescence images with 10X of magnification, and results were expressed as a percentage to control. Values represent mean ​± ​SD. Two-way analysis of variance and Tukey’s Multiple Comparison posthoc test was applied to all data. The p values are represented as followed: ∗∗∗p ​< ​0.001, ∗∗p ​< ​0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)