Table 2.
Antibodies used for flow cytometry analysis.
| Antibody | Dilution | Target cells | Host/isotype | Supplier |
|---|---|---|---|---|
| CD3-APC | 1:500 | T cells | Hamster IgG | BioLegend |
| CD4-BV605 | Helper T cells | Rat IgG2a, κ | BioLegend | |
| CD11b-BV421 | Myeloid cells | Rat IgG2b, κ | BioLegend | |
| CD45-A700 | Leukocytes | Rat IgG2b, κ | BioLegend | |
| F4/80-APC-Cy7 | Microglia/Macrophages | Rat IgG2a, κ | BioLegend | |
| Ly6C-FITC | Monocytes | Rat IgG2c, κ | BioLegend | |
| Ly6G-PE-Cy7 | 1:1000 | Neutrophils | Rat IgG2a, κ | BioLegend |
| FoxP3-PE-Cy5.5 | 1:500 | Regulatory T cells | Rat IgG2a, κ | eBioscience |