Fig. 2.

A20^+/− female mice show cognitive flexibility deficits at the age of 6 months.

(A) Mean distance to the acquisition (dark circles) and reversal (white circles) platforms for wildtype animals during reversal learning. On the first day, no significant preference for either the new nor the old location of the platform is seen; (B) In contrast, A20^+/− mice are swimming significantly closer to the old location (acq, black squares) of the platform; (C) Detailed analysis of the first day of reversal learning, shows that wildtype animals show a significant preference for the new location of the platform after four training sessions; (D) whereas A20^+/− mice fail to do so. (E) After five days of training, both WT and A20^+/− mice show a significant preference for the target quadrant and; (F) are swimming significantly closer to the reversal platform. All data are represented as mean±SEMs and statistical significance was determined by using two-way repeated measures ANOVA (A-E) or two-way ANOVA (F) using Sidak’s multiple comparisons test as post hoc analysis; (**P ​< ​0.01, ***P ​< ​0.001).